. Bulletin. La science. ASTRAGALUS JAEGERIANUS 161 DE LA DIVERSITÉ GÉNÉTIQUE. Fig. 1. Carte de la distribution connue de l'Astragalus jaegerianus. La zone d'étude est situé à environ 12 kilomètres au nord de Barstow, et est limité au nord et à l'Est et d'autre par ft. Réserve militaire et la Chine Irwin Lacs Centre d'armes navales. L'étendue de la gamme d'Astragalus jaegerianus est basée sur la charis (2002) rapport. Les localités sont délimitées par : O-ADN séquencé les individus seulement, O-AFLP génotypés individus seulement, -&amp ;-d'ADN séquencé et les deux individus génotypés AFLP. seulement en mesure d'isoler la dégradation D

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. Bulletin. Science. ASTRAGALUS JAEGERIANUS GENE DIVERSITY 161. Fig. 1. Map of the known distribution of Astragalus jaegerianus. The study area is located approximately 12 miles NNE from Barstow, and is bounded on the north and east sides by Ft. Irwin Military Reservation and China Lakes Naval Weapons Center. Extent of the range of Astragalus jaegerianus is based on the Charis (2002) report. Plant collecting localities are delineated by: O-DNA sequenced individuals only, O-AFLP Genotyped individuals only, -&-both DNA sequenced and AFLP Genotyped individuals. only able to isolate degraded DNA. Although we were able to amplify and sequence our loci of interest from this degraded DNA, the necrotic tissue did not provide sufficient high molecular weight DNA for generation of reliable AFLP markers. Thus, using the earlier collected tissue, nine individuals from each population were DNA sequenced, while later in the study ten individuals from each population were AFLP genotyped using green leaflet tissue collected in 2002-03 that yielded high molecular weight DNA; three individuals each that were collected within the Coolgardie, Brinkman Wash, and Goldstone populations were both DNA sequenced and AFLP genotyped. Two geographically widespread congeners (Astragalus didymocarpus H. & A. and A. layneae Greene) were also sampled within the range of A. jaegerianus (five of each species) for DNA sequencing to test the validity of the monomorphic sequence data for the 45 A. jaegerianus individuals. Genomic DNA isolation.—Three different methods were used for genomic DNA isolation from plant tissue-Qiagen's DNeasy Mini Plant Kit (Chatsworth, California USA), Epicentre's MasterPure™ Plant Leaf DNA Purification Kit (Madison, Wisconsin USA), and a 2X CTAB (hexadecyltrimethylammonium bromide) extraction protocol (Doyle and Doyle 1987). Extracted genomic DNA was quantified using spectrophotom- etry and visualized for high molecular weight DNA by agarose gel (0.8%) electroph

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