. Le bulletin biologique. Biologie ; zoologie ; biologie ; biologie marine. 142 C. C. ABRAHAM ET COLL. La figure 4. L'effet de dibromo-BAPTA sur les microtubules polaires dans ooplasm. Tous les œufs ont été fixées à /""  = 0,50. A et B sont des images obtenues à l'aide de la microscopie confocale à balayage laser microscope confocal Zeiss ; C et D ont été obtenues par microscopie à épifluorescence. Barre d'échelle  = 10 ^m ; A et B sont au même grossissement, C et D à l'autre. (A et B) de l'injection ni KC1 (A) ni dibromo-BAPTA (B ; fixe 32 min après la fécondation et environ 26 min après l'injection de dibromo-BAPTA) avait des appar

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. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 142 V. C. ABRAHAM ET AL. Figure 4. The effect of dibromo-BAPTA on microtubule arrays in polar ooplasm. All eggs were fixed at /"„ = 0.50. A and B are confocal images obtained using the Zeiss laser scanning confocal microscope; C and D were obtained by standard epifluorescence microscopy. Scale bar = 10 ^m; A and B are at the same magnification, C and D at another. (A and B) Injection of neither KC1 (A) nor dibromo-BAPTA (B; fixed 32 min after fertilization and about 26 min after injection of dibromo-BAPTA) had any apparent effect on the parallel array of microtubules in the vegetal mat. (C) Microtubules are oriented along meridian lines near the animal pole of this control (uninjected) egg. The pronuclei are to the right of the region shown in this photograph. (D) Injection of dibromo-BAPTA disrupted the radial pattern of microtubules in the blas- todisc. This egg was fixed about 52 min after fertilization and about 46 min after the injection of dibromo- BAPTA. Note the absence of radially oriented microtubules in this photograph, which is of the same region of the egg as the one shown in Fig. 4C. the polar regions of the egg. The striking feature of the region near the animal pole was the development of an array of microtubules that radiated from near the pro- nuclei to approximately 30° arc from the animal pole. Though microtubules were initially (T, , = 0.08) present near the animal pole as a sparse unoriented network, a dense network of radially oriented microtubules had formed by Tn = 0.3. Such radial arrays of microtubules are present in fertilized eggs of a number of animals, in- cluding annelids (Astrow el at., 1989). ctenophores (Hou- liston el a/., 1993), echinoderms (Harris el al, 1980), as- cidians (Sawada and Schatten. 1988), and amphibians (Houliston and Elinson, 1991; Schroeder and Card, 1992; Elinson and Palacek, 1993), and are associated with the sperm aster and sometimes w

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• bookauthorlilliefrankrat
• booksubjectbiology
• booksubjectzoology