. Le bulletin biologique. Biologie ; zoologie ; biologie ; biologie marine. 172 M. J. ELY ET A. M JUNGREIS groupe a été ensuite soumis à un 8,23 kr L'exposition aux rayons X (325 r/min) 24 heures après la deuxième administration l'ecdysone. Jour 1 Les animaux recevant 4 p.g ecdysone/gramme de poids sans irradiation subséquente a servi de groupe de contrôle. En second lieu, jour 12 animaux ont été traités de façon identique à celui utilisé pour le jour 1 les animaux à l'exception que l'ED0 X-irradiation exposition était de 25 kr (325 r/min). En troisième lieu, jour 4 les animaux ont été traités d'une manière similaire à jour 1 les animaux sauf qu'à 6

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. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 172 M. J. ELY AND A. M JUNGREIS group was then subjected to an 8.23 kr X-ray exposure (325 r/minute) 24 hours following the second ecdysone administration. Day 1 animals receiving 4 p.g ecdysone/gram wet weight without subsequent irradiation served as a control group. Secondly, day 12 animals were treated in a manner identical to that used for day 1 animals with the exception that the ED0 X-irradiation exposure was 25 kr (325 r/minute). Thirdly, day 4 animals were treated in a manner similar to day 1 animals except that at 6, 12. 24, 36, and 48 hours post ecdysone injections the animals were irradiated at an ED0 level. Fourthly, day 4 animals were X-irradi- ated with an exposure of 8.23 kr (325 r/minute) 24 hours following administration of 2. 4, 6, 12. and 16 micrograms of ecdysone per gram of wet weight. Eclosion success was determined for each experimental group and expressed as a percentage of eclosion success observed in noninjected animals irradiated at similar time intervals. Day 4 and day 12 data were analyzed using single classi- fication analysis of variance. The Student-Newman-Keuls test was employed for a comparison among means (Sokal and Rohlf, 1969). RESULTS Effects of X-irradiation on eclosion The effect of X-irradiation on eclosion of M. se.vta during the pupal-adult trans- formation was investigated by initially determining the ED50 for animals on each day of development and by utilizing the ED50 values as a relative measure of radiation sensitivity (Fig. 1). An initial period of nearly constant radiosensitivity 80- z o u uj 40' Ul a. i Z O 20 -o—0_o—o—Q. I I I I ' 12 16 20 DAYS AFTER LARVAL-PUPAL ECDYSIS FIGURE 1. ED50 values (X-ray exposures required to prevent eclosion in 50% of the irradiated animals) during pupal-adult development of Manduca sc.vta.. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readabilit

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• bookauthorlilliefrankrat
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• booksubjectzoology