. Le bulletin biologique. Biologie ; zoologie ; biologie ; biologie marine. 400 500 600 longueur d'onde (nm) Figure 7. Les spectres de luminescence coelenterazine catalysée par pu- luciférase appose Periphylla-L (ligne pleine), la luciférase purifiée-O (ligne en pointillé), et une préparation de la luciférase-O (ligne pointillée) en 20 mM Tris-HCl, pH 7,8, contenant du NaCl 1 M et 0,05  % de la surface corporelle. L'intensité de luminescence de coelenterazine catalysée par la luciférase était affecteii par la concentration de coelen- terazine jusqu'à 3 /xA/, comme illustré à la Figure 8. À partir des données de la figure, la constante de Michaelis de cette enzyme est f

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. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 400 500 600 Wavelength (nm) Figure 7. Luminescence spectra of coelenterazine catalyzed by pu- rified Periphylla luciferase-L (solid line), purified luciferase-O (broken line), and a crude preparation of luciferase-O (dotted line) in 20 mM Tris-HCl buffer, pH 7.8, containing 1 M NaCl and 0.05% BSA. The luminescence intensity of coelenterazine catalyzed by luciferase was affecteii by the concentration of coelen- terazine up to 3 /xA/, as shown in Figure 8. From the data in the figure, the Michaelis constant of this enzyme is fluorescent substances with corresponding emission max- ima. A previously reported observation that the clusters of the grains in the exumbrellar photocytes. but not the grains in the cortex layer of eggs, show a pinkish fluores- cence that rapidly fades under UV light (Flood et al.. 1996) is apparently not in accord with the present results. One explanation for the discrepancy is that a pink fluo- rescent substance is more abundant in the lappet pho- tocytes than in the ovarian photocytes, but that the fluo- rescent substance in lappets easily dissociates from lucif- erase-L molecules during the extraction process, whereas that in eggs does not dissociate from luciferase-O. In our actual experimental procedure, the lappet fluorescent substance might have dissociated from luciferase-L, even when the frozen lappets were thawed and the cells were broken. In any event, the bioluminescence spectrum of Periphylla is probably not affected significantly by these fluorescent substances, because the bioluminescence spectrum of live Periphylla was previously reported to be a single peak with a maximum at 470-475 nm (Her- ring, 1983; Widder et al., 1983).. 0 12 3 Concentration of coelenterazine (^jM) Figure 8. Effect of coelenterazine concentration on the intensity of luminescence when the reaction was catalyzed by Periphylla luciferase- L. The concentration of coelenterazine represents th

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