. Bactériologie agricoles ; une étude de la relation de Bagnères-de-vie à la ferme, avec des expériences en laboratoire pour les étudiants, les micro-organismes du sol, les engrais, les eaux usées, l'eau, les produits laitiers, divers produits agricoles et des maladies des animaux et des plantes. Bactériologie, de l'agriculture. L'ANTHRAX OU LA FIÈVRE SPLÉNIQUE 303 démontré vraiment comme la cause de la maladie jusqu'en 1875, par les travaux de Koch, et peu de temps après par Pasteur (Fig. 51). Après environ vingt-quatre ans de litige, la dernière démonstration est due à des expériences telles que les suivantes. Il est facile de montrer par le microscope qui this.orga

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. Agricultural bacteriology; a study of the relation of germ life to the farm, with laboratory experiments for students, microorganisms of soil, fertilizers, sewage, water, dairy products, miscellaneous farm products and of diseases of animals and plants. Bacteriology, Agricultural. ANTHRAX OR SPLENIC FEVER 303 really demonstrated as the cause of the disease until 1875, by the work of Koch, and shortly afterward by Pasteur (Fig. 51). After some twenty-four years of dispute the final demonstration was due to such experiments as the following. It was easy to show by the microscope that this.organism is present in the blood of the animals suffering from the disease, and that a drop of such blood, containing the organism, when injected into healthy animals would inevitably produce the disease in the inoculated animals. But this did not necessarily prove their causal agency, for it was possible to claim that there were some other poisons in such blood. For final proof it was necessary to separate the bacteria from the drop of blood, cultivate them, and inoculate animals. Fig. ST.—B. anthracis, the cause of splenic fever. with the pure cultures. At the time that this disease was first being studied no methods were known of obtaining isolated bacteria in pure cultures, and hence the long dispute. Pasteur finally procured his results as follows. Finding that the bacterium would grow in a solution made by steeping yeast in water, Pasteur inoculated a sterile flask of such yeast-water with a drop of anthrax blood. In a day or two his flask was filled with bacteria which had arisen from the first by division. The inoculation of a second flask from the first showed like results, and by con- tinuing such inoculations from flask to flask he rapidly got rid of all parts of the original drop of blood, except such parts as had been multiplying in the flasks. His microscope showed him that. Please note that these images are extracted from scanned page images that may have been digi

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