. Les principes de la bactériologie : un manuel pratique pour les étudiants et les médecins. terilization complète. Les tubes arethen retiré et mis à refroidir. Le coton utilisé à cette fin doit être l'ordi-nary molleton de coton des magasins, et non pas absorbentcotton ; celui-ci devient trop étroitement emballé, et est en outre,,beaucoup trop cher à cette fin. Il faut veiller à ne pas brûler le coton, autre que les tubes seront recouvertes d'une couleur sombre, empyreumatique,-huileuse, ce qui nécessite des recleans-ing. Le remplissage des tubes.-lorsque les tubes sont coldthey peut être comblé. C'est meilleur le cac

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. The principles of bacteriology: a practical manual for students and physicians. terilization complete. The tubes arethen removed and allowed to cool. The cotton used for this purpose should be the ordi-nary cotton batting of the shops, and not absorbentcotton; the latter becomes too tightly packed, and is, moreover, much too expensive for this purpose. Care should be taken not to burn the cotton, other-wise the tubes will become coated with a dark-colored, empyreumatic, oily deposit, which necessitates recleans-ing. Filling the Tubes.—When the tubes are coldthey may be filled. This is best accomplished by the 128 BAGTERIOLOGY. use of a separating funnel, such as is shown in Fig.22. The liquefied medium is poured into this funnel, which has been carefully washed, and by pressing thepinchcock with which the funnel is provided the desiredamount of material (6-10 c.c.) may be allowed to flowinto the tubes held under its opening. It is not neces-sary to sterilize the funnel, for the medium is to be sub-jected to this process as soon as it is in the test-tubes. Fig. 22.. Funnel for filling tubes with culture-media. Care should be taken that none of the medium isdropped upon the mouth of the test-tube, otherwise thecotton plug becomes adherent to it, and is not onlydifiicult to remove, but presents a very untidy appear-ance and interferes materially with the manipulations. FILLING THE TUBES. 129 As soon as the tubes have been filled they are to besterilized in the steam sterilizer for fifteen minutes oneach of three successive days. During the interveningdays they may be kept at the ordinary room-tempera-ture. When sterilization is complete and the medium inthe tubes is still liquid, some of them may be placed ina slanting position, at an angle of about ten degreeswith the surface on which they rest, and the mediumallowed to solidify in this position. These are for theso-called slant-cultures. The remainder may solidify inthe erect position; these serve for making plates.

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• bookcentury1900
• bookdecade1900
• booksubjectbacteri
• bookyear1902